Fig. 4

HVJ-E induced autophagy in HeLa cells in a PI3K/AKT/mTOR-dependent manner. HeLa cells were cultured with HVJ-E at the indicated MOI. a–c Expression levels of p-mTOR, p-70S6K, Akt, and Beclin 1 were detected by Western blot. d, e Cells were pretreated with 740Y-P or SC79 prior to infection. Autophagosomes in cells were detected by TEM. f Cells were pretreated with 740Y-P prior to infection. The expression of LC3 was assessed by Western blot. β-actin was used as a loading control. *P < 0.05, **P < 0.01