Fig. 5
From: Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells
Overexpression of miRNA344a-3p induced apoptosis in curcumin-treated RT4 schwannoma cells. a miRNA 344a-3p mimic attenuated the mRNA expression of Bcl2 while increasing that of Bax. qPCR was performed after curcumin treatment in RT4 schwannoma cells for 24 h. 18S was used as an internal control. Data are presented as the mean ± SD of triplicate samples. ***p < 0.001. b Curcumin treatment in RT4 schwannoma cells downregulated the mRNA level of Bcl2 while enhancing that of Bax. c The effect of the miRNA 344a-3p mimic combined with curcumin treatment in RT4 schwannoma cells on caspase-3 and caspase-9 expression by Western blot analysis. Bar graphs represent the relative expression of procaspase-9 or cleaved caspase-3 to β-actin determined using ImageJ software. Data are presented as the mean ± SD of triplicate samples. ***p < 0.001, **p < 0.01, and *p < 0.05. d Effect of the miRNA 344a-3p inhibitor combined with curcumin treatment in RT4 schwannoma cells on cleaved caspase-3 and caspase-9 expression by Western blot analysis