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Fig. 3 | Cancer Cell International

Fig. 3

From: miRNA-133b targets FGFR1 and presents multiple tumor suppressor activities in osteosarcoma

Fig. 3

miR-133b and FGFR1 essentially regulated the proliferation and apoptosis of OS cells. The viability of MG-63 (a) and U2OS cells (b) transfected with miR-133b mimics vs. miR-133b NC, or with shFGFR1 vs. shNC for 24, 48, and 72 h, respectively, was examined by MTT assay. c The cell-cycle distribution of MG-63 (upper panels) and U2OS cells (lower panels) transfected with miR-133b mimics vs. miR-133b NC, or with shFGFR1 vs. shNC was determined by flow cytometry analysis. d The quantification of the percentage (%) of cells in different phases of cell cycle (G1, S, G2/M) was presented. e Apoptosis was examined by staining the indicated cells with Annexin V and PI. f The total  % of early apoptotic (Annexin C+PI) and late apoptotic (Annexin V+PI+) cells were quantified from at least three independent experiments. *P < 0.05 and ***P < 0.001

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