Fig. 2

Effect of AdoMet, miR-34a and miR-34c mimic combination on apoptotic process in MCF-7 cells. Cells were not treated (control) or treated with 500 μM AdoMet and/or 100 nM miR-34a and miR-34c mimic for 72 h. a Apoptosis was evaluated by FACS analysis. The different quadrants report the percentage of cells: viable cells, lower left (Q4); early apoptotic cells, bottom right (Q3); late apoptotic cells, top right (Q2); and non-viable necrotic cells, upper left (Q1). The experiment was repeated three times and the results were always similar. b Quantification of apoptosis evaluated by the FACS analysis. The histogram plot shows the percentage apoptotic cells for single treatment. The analysis was the average of at least three independent experiments. c Western blot assay of MCF-7 cell extracts was evaluated for the expression of pro-caspase 9, pro-caspase 8, pro-caspase 6, and PARP. For the equal loading of protein in the lanes, the expression of the house-keeping protein α-tubulin was used as a standard. The images are representative of three immunoblotting analyses obtained from at least three independent experiments