Fig. 3

Effect of AdoMet and miR-34a mimic or miR-34c mimic on the HDAC1, SIRT1 and p53 expression levels. a Alignment of miR-34a and miR-34c with HDAC1 3′UTR obtained from miRNA-mRNA integration analysis using the TargetScan microRNA target prediction software. b Cells were transfected with miR-34a and miR-34c mimic or inhibitor, in the presence or not (Control) of 500 μMAdoMet for 72 h. Then, 10 μg of cell lysates were subjected to SDS-PAGE, incubated with antibodies against the indicated proteins and analyzed by Western blotting. The housekeeping protein α-tubulin was used as loading control. Graphs show the densitometric intensity of Ac-p53/p53 band ratio. The intensities of signals were expressed as arbitrary units. The images are representative of three immunoblotting analyses obtained from at least three independent experiments. Bars, SDs