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Fig. 5 | Cancer Cell International

Fig. 5

From: S-Adenosylmethionine regulates apoptosis and autophagy in MCF-7 breast cancer cells through the modulation of specific microRNAs

Fig. 5

Effect of AdoMet and miR-486-5p mimic or inhibitor combination on the autophagy process. Cells were not treated (Control) or treated with 500 μM AdoMet and/or transfected with miR-486-5p mimic or miR-486-5p inhibitor for 72 h. a Cells were labeled with anti-LC3B antibody and then analyzed by flow cytometry after staining with goat anti-rabbit IgG (Alexa Fluor647). The percentage of MFI was calculated as % of untreated control. Values are the means of three independent experiments. b Western blot assay for the expression of LC3B, Beclin1 and ATG7. c Graphs show the densitometric intensity of Beclin 1 and ATG7 bands, and LC3BII/I bands ratio. The intensities of signals were expressed as arbitrary units. For the equal loading of protein in the lanes, β-actin was used. Bars, SDs

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