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Fig. 6 | Cancer Cell International

Fig. 6

From: S-Adenosylmethionine regulates apoptosis and autophagy in MCF-7 breast cancer cells through the modulation of specific microRNAs

Fig. 6

Effect of AdoMet and miR-486-5p mimic or inhibitor on PTEN/AKT signaling pathway. a Alignment of miR-486-5p with PTEN 3′UTR obtained from miRNA-mRNA integration analysis using the TargetScan microRNA target prediction software. b Cells were transfected with miR-486-5p mimic or inhibitor, in the presence or not (Control) of 500 μMAdoMet for 48 and 72 h. Then, 10 μg of cell lysates were subjected to SDS-PAGE, incubated with antibodies against the indicated proteins and analyzed by Western blotting. The housekeeping protein β-actin was used as loading control. Graphs show the densitometric intensity of pAKT/AKT bands ratio. The intensities of signals were expressed as arbitrary units. Bars, SDs

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