Fig. 5
From: TRIM14 promotes colorectal cancer cell migration and invasion through the SPHK1/STAT3 pathway
TRIM14 promoted migration and invasion of CRC cells through activating the SPHK1/STAT3 signaling. LoVo cells were transduced with pLVX-TRIM14 or pLVX, and treated with 5 μM SKI-II (Selleck Chemicals, Houston, TX, USA), 30 μM AG490 (Selleck Chemicals) or vehicle (DMSO). a Transwell migration and invasion assays were performed. The stained cells were counted in five random fields at ×200 magnification, and the average number was taken. The average number of Control group (cells transduced with pLVX and treated with DMSO) was set as 100%, and relative migration and invasion of other groups was calculated by comparison of the cell number with the average number of control group. ***P < 0.001 versus pLVX + DMSO; ###P < 0.001 versus pLVX-p53 + DMSO. b The levels of SPHK1, p-STAT3, STAT3, VEGF, MMP2 and MMP9 were detected