Fig. 3

The effects of silencing TRIP13 on cell viability and cell apoptosis in HepG2 and MHCC97H cells. a Silencing TRIP13 decreased the mRNA expression of TRIP13, which was performed by qRT-PCR in HepG2 cells. b Silencing TRIP13 decreased the protein expression of TRIP13, which was performed by western blot in HepG2 cells. GAPDH served as an internal control. c Cell viability was performed by CCK-8 assay at 12, 24 and 48 h in HepG2 cells. d Flow cytometry was used to detect the effect of silencing TRIP13 on cell apoptosis in HepG2 cells. e Apoptosis rate was shown as bar diagrams in HepG2 cells. f Silencing TRIP13 decreased the mRNA expression of TRIP13, which was performed by qRT-PCR in MHCC97H cells. g Silencing TRIP13 decreased the protein expression of TRIP13, which was performed by western blot in MHCC97H cells. GAPDH served as an internal control. h Cell viability was performed by CCK-8 assay at 12, 24 and 48 h in MHCC97H cells. i Flow cytometry was used to detect the effect of silencing TRIP13 on cell apoptosis in MHCC97H cells. j Apoptosis rate was shown as bar diagrams in MHCC97H cells. Data were expressed as mean ± SD from three independent experiments (*compared with control or mock, *P < 0.05, **P < 0.01)