Fig. 5

The effects of silencing TRIP13 on the expressions of related genes in HepG2 and MHCC97H cells. qRT-PCR was performed to detect the mRNA expressions of Ki67 (a), TIMP-2 (b) and MMP-2 (c) in HepG2 cells. d Western blot was performed to assess the protein expressions of Ki67, active-caspase-3, TIMP-2 and MMP-2 in HepG2 cells. e Relative protein levels of Ki67, active-caspase-3, TIMP-2 and MMP-2 were shown as bar diagrams in HepG2 cells. f Western blot was performed to assess the protein expressions of TGF-β1, TβRII, p-smad3 and smad3 in HepG2 cells. Relative protein expressions of TGF-β1 (g), TβRII (h) and p-smad3/smad3 (i) were shown as bar diagrams in HepG2 cells. qRT-PCR was performed to detect the mRNA expressions of Ki67 (j), TIMP-2 (k) and MMP-2 (l) in MHCC97H cells. m Western blot was performed to assess the protein expression of Ki67, active-caspase-3, TIMP-2 and MMP-2 in MHCC97H cells. n Relative protein levels of Ki67, active-caspase-3, TIMP-2 and MMP-2 were shown as bar diagrams in MHCC97H cells. o Western blot was performed to assess the protein expressions of TGF-β1, TβRII, p-smad3 and smad3 in MHCC97H cells. Relative protein expressions of TGF-β1 (p), TβRII (q) and p-smad3/smad3 (r) were shown as bar diagrams in MHCC97H cells. GAPDH served as an internal control. Data were expressed as mean ± SD from three independent experiments (*compared with control or mock, *P < 0.05, **P < 0.01)