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Fig. 1 | Cancer Cell International

Fig. 1

From: Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein

Fig. 1

Anticancer activity of ABF in MCF-7 cells in vitro. a The concentration–response curves of ABF in MCF-7 cells. Cell viability of MCF-7 cells treated with different concentrations of ABF for 24 h, 36 h, 48 h or 72 h was measured using an MTT assay. Data are shown as the mean ± SEM (n = 3). b ABF induces apoptosis in a time- and concentration-dependent manner. Following treatment, cell apoptosis was analyzed by Annexin V-FITC/PI staining assay. Data represents the mean ± SEM (n = 3). **P < 0.01, and ***P < 0.001 versus the control. c Apoptotic effect of ABF was determined by a TUNEL assay. TUNEL (green) was used to mark fragmented DNA. DAPI (blue) was used to indicate the cell nuclei. Original magnification: 200× ; Scale bar: 200 μm. d The quantitative data of the TUNEL assay. Data represent the mean ± SEM, n = 3. ***P < 0.001 versus the control. e Effects of ABF on the expression of apoptosis-related proteins. Whole-cell lysates extracted from MCF-7 cells treated with ABF (20 nM) were evaluated by Western blotting. β-actin was used as the loading control. f Quantitative data of the protein levels described above. Data represent the mean ± SEM, n = 3. ***P < 0.001 versus the control

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