Fig. 4

JNK signaling is involved in ABF-induced YAP phosphorylation. a LAST1 was not involved in YAP shift induced by ABF. Whole cell lysates were extracted from MCF-7 cells treated with ABF (20 nM) in the presence or absence of LAST1 siRNA. The lysates were evaluated by Western blotting. b Quantitative results for the shifting of YAP were analyzed. Data represent the mean ± SEM, n = 3. ^***P < 0.001 versus the control. c ABF increased the level of p-JNK in MCF-7 cells. Lysates from cells treated with or without ABF were evaluated by Western blotting with the indicated antibodies. d Quantitative results of relative protein expression were analyzed. Data represent the mean ± SEM, n = 3, ^***P < 0.001 versus the control. e Effects of different kinase inhibitors on YAP shifting induced by ABF. Following treatment with ABF (20 nM) in the presence or absence of SP600125, SB203580 and U0126, cell lysates were collected and subjected to Western blotting. f Quantification of the YAP shift was analyzed by ImageJ software. Data represent the mean ± SEM, n = 3. ^***P < 0.001 versus the control; ^###P < 0.001 versus the ABF group