Fig. 2
Neuropeptides induced the invasiveness of MDA-MB-231LUC+ cells using a a vertical 3D invasion assay. a Representative images of the inserts containing the invasive cells at 72 h after substance P (100 nM), CGRP (100 nM), NKA (50 nM) and NP combination (Comb), visualized by transmitted light microscopy. Scale bar = 100 µm (bottom panel). b NP time-dependent induced invasion of human metastatic breast cancer cells. NP and its combination increased the invasion of metastatic breast cancer cells after 24, 48 and 72 h (top, center and bottom panel, respectively). Three to five independent experiments (each with duplicated samples) were performed. Data are presented as median (numbers and horizontal line) with boxes representing the 25 and 75 percentiles, *P < 0.05; **P < 0.01; ***P < 0.001 compared to control treatment