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Fig. 3 | Cancer Cell International

Fig. 3

From: Targeting cellular metabolism using rapamycin and/or doxycycline enhances anti-tumour effects in human glioma cells

Fig. 3

The anti-proliferative effects and alterations in mTOR activity and other metabolism related protein levels in response to mTORI and temozolomide treatments in human glioma cell lines. a mTOR inhibitors (rapamycin—Rapa 50 ng/mL; NVP-BEZ235—BEZ 1 µM; PP242 1 µM) and temozolomide (TMZ 100 µM) 72-h combination treatments effectively inhibited the proliferation of U251, U87 and U373-U cells—Alamar Blue and SRB proliferation test results, the cell proliferation of untreated controls were considered 100% (temozolomide slightly reduced the proliferation and had no significant growth inhibitory effect on U251, mTORIs had similar effects as in Fig. 1a, and all temozolomide + mTORI combined treatments had significant anti-proliferative effects, p < 0.05; the additive (A) or synergistic (S) effects of inhibitor combinations were given based on CI calculation, SD was added); b altered expressions of mTORC1 and C2 activity related proteins and other metabolic enzymes were also shown after different mono-treatments (results of representative Western blots after 72-h treatments)

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