Fig. 4

Metabolic drugs induced proliferative and protein expression profile changes in human glioma cells. a Doxycycline (Doxy 10 µM), etomoxir (Etom 50 µM), chloroquine (Chl 50 µM) 72-h treatments have slight growth inhibitory effects in U251, U87 and U373-U (Alamar Blue proliferation test data; *p < 0.05); b ACSS2, FASN, CPT1a and p-(Ser473)-Akt protein expressions were influenced by rapamycin, doxycycline, etomoxir and chloroquine after 72-h mono-treatments; c FASN, β-F1-ATPase, p-S6, p-(Ser473)-Akt and CPT1a protein expression compared to the untreated controls after applying two-drug combinations in U251 glioma cells (50 ng/mL rapamycin + 100 µM temozolomide—Rapa + TMZ; 50 ng/mL rapamycin + 10 µM doxycycline—Rapa + Doxy; 100 µM temozolomide + 10 µM doxycycline—TMZ + Doxy (Western blot results)—representative Western blot figures