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Fig. 5 | Cancer Cell International

Fig. 5

From: Targeting cellular metabolism using rapamycin and/or doxycycline enhances anti-tumour effects in human glioma cells

Fig. 5

Rapamycin and temozolomide combined with other metabolic inhibitors have different in vitro anti-proliferative effects in human glioma cell lines. Alamar Blue test results after 72-h different two-drug combined in vitro treatments in U251 (a), U87 (b) and U373-U (c) glioma cells (rapamycin—Rapa 50 ng/mL, temozolomide—TMZ 100 µM, doxycycline—Doxy 10 µM; etomoxir—Etom 50 µM; chloroquine—Chl 50 µM). The additive (A) or synergistic (S) effects of combinations were given based on CI calculation, SD was added, the anti-proliferative effects were significant (p < 0.05) in almost all combined treatments compared to untreated cultures except for doxycycline + etomoxir and temozolomide + etomoxir treatments

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