Fig. 7From: Targeting cellular metabolism using rapamycin and/or doxycycline enhances anti-tumour effects in human glioma cellsRepresentative stainings for metabolic protein expression analyses in human glioma biopsies. Metabolic and mTOR pathway related protein expression patterns and evaluated staining scores were shown in representative human IDH wild-type and mutant gliomas. Stainings of normal brain tissues were added to the upper left corner. High mTOR activity—with tissue heterogeneity and characteristic CPT1a protein overexpression (17/18 3+ scores) were detected in almost all studied gliomas. The intra- and intertumoral heterogeneity appeared in e.g. p-S6, FASN and p-(Ser473)-Akt stainings, respectively. Significant differences between IDH wild-type and mutant cases were only observed in GLS expression scores, the IDH-mutant glioma tissues expressed higher amount of GLS protein (expression scores were 2/3+ in 6/8 mutant and 0/1+ in 8/10 studied IDH wild-type cases). Further explanation is in the text. Immunohistochemistry stainings of IDH wild-type (n = 10) and IDH-mutant (n = 8) cases were developed by DAB (brown) using haematoxylin counterstaining—the representative photos were taken by Panoramic Viewer (3D Histech—×40)Back to article page