Fig. 1

Hypermethylation of the CpG island in the SPOP gene promoter in NSCLC. a Scheme of the location of the CpG islands in the promoter of the SPOP gene. The CpG sites are indicated by vertical red lines. The regions used for MS-PCR and BSP are also indicated. b Representative results of methylation analysis by MS-PCR in NSCLC tissues (T) and adjacent normal lung tissues (N). U unmethylation; M, methylation. c Statistical results showing that the methylation level in the NSCLC tissues was higher than that in the adjacent normal tissues. d Correlation analysis of the MSP results. *P < 0.05. Data represent the results of three independent experiments