Fig. 2From: Speckle-type POZ protein functions as a tumor suppressor in non-small cell lung cancer due to DNA methylationHypermethylation of specific CpG sites within the SPOP gene promoter inhibits transcriptional activities. a Schematic of the regions used for BSP showing the locations of the 17 CpG sites in the SPOP gene promoter area. b Bisulfite sequencing analysis of the methylation status of the SPOP gene promoter. Each oval indicates clones from different cell lines. Eight clones were subjected to bisulfite sequencing. The methylated clones of individual CpG sites are labeled in black. c The mRNA levels of SPOP in different cell lines. d Treatment with two luciferase constructs with SssI methylase in vitro. *P < 0.05. Data represent the results of three independent experimentsBack to article page