Fig. 5

SPOP mediates C/EBPα-regulated proliferation, migration and invasion of lung cancer cells in vitro and in vivo. a C/EBPα and SPOP expression in A429 and H1299 cells treated as indicated was detected by western blotting. b Cell proliferation ability was assessed by MTT assays in A429 and H1299 cells treated as indicated. c, d Cell Migration and invasion abilities were assessed using Transwell and Matrigel-coated Transwell assays, respectively, in A427 (c) and H1299 (d) cells treated as indicated. The data are presented as the mean ± SD of three independent experiments. *P < 0.05 versus A427 or H1299 cells cotransfected with siRNA and vector control (siControl + Vector); ^#P < 0.05 versus A427 cells cotransfected with siSPOP and vector control (Vector + siSPOP) or H1299 cells cotransfected with siControl and SPOP expression plasmid (siControl + SPOP). e Representative xenograft tumors in nude mice (upper left panel), and representative photographs of tumors removed from bodies 28 days after implantation (bottom left panel). f Tumor growth curves showing that the C/EBPα depletion significantly enhanced xenograft tumor growth, and these effects were reversed by the SPOP overexpression in the nude mice. *P < 0.05 versus siRNA and vector control group; ^#P < 0.05 versus siSPOP and vector control group