Fig. 4

The cell cycle distribution, p21 and p27 expressions were determined in siESPN ESCC cells. a The cell cycle progression was analyzed by flow cytometry in negative control, ESPN-depleted KYSE270 and ESPN-depleted TE10 cells. b The protein expression levels of p21 and p27 were examined by Western blotting in negative control, ESPN-depleted KYSE270 and ESPN-depleted TE10 cells