Fig. 6

ESE-15-one and ESE-one increase FAK phosphorylation in G1/S arrested MDA-MB-231 cells. a Western blot analysis of interphase arrested cells exposed to ESE-15-one or ESE-one was performed with antibodies directed against phosphorylated Y397 FAK or total FAK. The average of 3 experiments were quantified using densitometry and relative FAK phosphorylation was calculated by dividing the phosphorylated FAK levels by the total FAK levels. The graph shows the average with error bars representing SD. *P-value < 0.05 when compared to the DMSO control. b Peptide pulldown analysis of active RhoA GTPase visualized by western blot. Cells were treated with DMSO, ESE-15-one or ESE-one as above before active RhoA was precipitated. Western blot was performed on precipitated active RhoA and total cellular RhoA levels. The average of 3 independent experiments were quantified using densitometry and relative RhoA activity was calculated by dividing the active precipitated RhoA levels by the total RhoA levels. The graph shows the average with error bars representing SD. *P-value < 0.05 when compared to the DMSO control