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Fig. 3 | Cancer Cell International

Fig. 3

From: SENP1-mediated deSUMOylation of USP28 regulated HIF-1α accumulation and activation during hypoxia response

Fig. 3

SUMOylation of USP28 impaired its deubiquitinating activity on HIF-1α. 293T cells were co-transfected with Flag-USP28 WT or Flag-USP28 K99R, His-ubiquitin, FBXW7 and HA-HIF-1α for 36 h and MG132 was added 4 h before cells were harvested. Ubiquitinated HIF-1α was purified from 293T cells by using anti-HA antibody and detected by western blot with anti-ubiquitin antibody

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