Fig. 3

Effects of selumetinib and erlotinib single and combined drug treatments in four MEKi-Re LGSC cell lines. The graphed curves (a) represent the results from the proliferation experiments and the bar graphs (b) represent the results from the viability (MTS and CV) assays performed at the end of the proliferation experiments. The photomicrographs (c) show IncuCyte™ images at the completion of the experiment. All four cell lines were resistant to single selumetinib and erlotinib treatments. However, when the drugs were combined, VOA-6406 and VOA-3723 cell lines demonstrated complete cell death while VOA-4627 and VOA-3993 cells were shown to be resistant to the dual selumetinib and erlotinib treatment combination