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Table 1 Cas9 proteins and their function in CRISPR editing

From: CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics

Cas protein CRISPR system Guide RNA Nuclease activity Recognition sequence on target Reference
Cas9 Type II crRNA + transcrRNA Yes G-rich PAM [49, 50]
Cpf1 Type V crRNA Yes G-rich PAM [46]
Cas13a Type VI-A crRNA + transcrRNA Yes Except G-rich [54]
dCas9 Type II crRNA + transcrRNA No G-rich PAM [140]
Cas3 Type I crRNA Yes T-rich PAM [141, 142]
Cas10/Csm1 Type III crRNA Yes AT-rich PAM [143, 144]
dCas13a Type VI-A crRNA + transcrRNA Yes Except G-rich [145]