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Fig. 2 | Cancer Cell International

Fig. 2

From: Long non-coding RNA CASC2 upregulates PTEN to suppress pancreatic carcinoma cell metastasis by downregulating miR-21

Fig. 2

MiR-21 overexpression reversed the role of CASC2 in PANC-1 pancreatic carcinoma cells. a Expression of miR-21 in the human pancreatic cancer cell lines and normal pancreatic HPDE6-C7 cells. b PANC-1 cells were transfected with the CASC2-expressing vector (pEX-CASC2) or a negative control (pEX) for 48 h, and the expression of miR-21 was detected. c The wild-type or mutant CASC2 with the miR-21-binding site was generated, integrated into a luciferase vector to form the reporter vectors, and cotransfected with miR-21 mimics or miR-21 mimic NC (NC) into the cells by the Lipofectamine 3000 method. Dual-luciferase reporter assay showed that miR-21 was a direct target of CASC2. Then, PANC-1 cells were transfected with the CASC2-expressing vector (pEX-CASC2) or the corresponding negative control (pEX) and miR-21 mimics or the corresponding negative control (mimics NC) for 48 h. d The expression of miR-21 was detected. e Cell migration was detected. f Cell invasion was detected. *P < 0.05; **P < 0.01; ***P < 0.001. Scale bar: 100 μm

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