Fig. 3

CCNB1 knockdown inhibits HCC cell migration and invasion. a HepG2 and SMMC-7721 cells were transfected with either Scramble-siRNA or CCNB1 siRNA at 50 nM. Micrographs of HepG2 and SMMC-7721 cells at 0 h and 48 h after wounding. b Migration distance of HepG2 and SMMC-7721 cells for 48 h was calculated and bar graphs represent the mean ± SD from 3 independent experiments. ^**p < 0.01 vs. NS-siRNA. c, d Transwell Cell invasion activity was evaluated by Matri-gel invasion assay. The number of invading cells was counted in three images per membrane by microscopy using a ×20 objective, scale bar = 100 μm. Experiments were performed in triplicate and repeated at least three times. **p < 0.01 vs. NS control