Fig. 3
RZM upregulated the expression of p21 through the activation of AMPK. a RT-qPCR analysis of relative gene mRNA expressions in RZM-treated HepG2 cells. Transcript levels were normalized to GAPDH expressions (*P < 0.05 and **P < 0.01 versus the control group). b, c sh-p21 or/and RZM regulated the expression of p21 were assayed by RT-qPCR and western blotting assays (##P < 0.01 and *P < 0.05 versus the control group). d MTS assay conducted in HepG2 cells under the corresponding conditions. Values shown are mean ± SD of triplicate measurements (**P < 0.01 and #P < 0.05 versus the control group). e Western blotting analysis of the activation of AMPK in HepG2 and SK-hep1 cells, using GAPDH as an endogenous control. f Relative protein expression of AMPK, p-AMPK and p21 in cells treated with RZM or/and compound C. GAPDH was used as an internal control