Fig. 5
From: Long noncoding RNA PXN-AS1-L promotes non-small cell lung cancer progression via regulating PXN
PXN-AS1-L up-regulated PXN expression. a PXN mRNA levels in PXN-AS1-L stably overexpressed and control A549 cells were detected by qPCR. b PXN mRNA levels in PXN-AS1-L stably depleted and control NCI-H1299 cells were detected by qPCR. c PXN protein levels in PXN-AS1-L stably overexpressed and control A549 cells were detected by western blot. d PXN protein levels in PXN-AS1-L stably depleted and control NCI-H1299 cells were detected by western blot. e After transient overexpressing PXN-AS1-L in A549 cells, the stability of PXN mRNA over time was determined after blocking new RNA synthesis with α-amanitin (50 µM) and normalized to 18S rRNA (a product of RNA polymerase I that is unchanged by α-amanitin). f After transient depleting PXN-AS1-L in NCI-H1299 cells, the stability of PXN mRNA over time was determined after blocking new RNA synthesis with α-amanitin (50 µM) and normalized to 18S rRNA. Results are shown as mean ± SD of three independent experiments. **P < 0.01, ***P < 0.001 by Student’s t-test