Establishment and characterization of a patient-derived circulating lung tumor cell line in vitro and in vivo

Table 1 CTCs counts, isolation and ex vivo expansion

Patient ID	CTC detection by one-step microfluidics-based immunomagnetic isolation		Ex vivo CTC culture
N = 89	N = 4	N = 85	N = 2
1		22
2		3
3		81
4		49
5		1
6		28
7		147
8		10
9		2
10		22
11		8
12		1
13		143
14		11
15		39
16		15
17		6
18		22
19		21
20		33
21		4
22		3
23		212	Yes
24		18
25		9
26		5
27		7
28		4
29		227
30		57
31	0
32		13
33		1
34		25
35	0
36		139
37		12
38		1
39		3
40		8
41		26
42		5
43		6
44		2
45		3
46		130	Yes^a
47		5
48		18
49		16
50		6
51		187
52		5
53		11
54		102
55		177
56		17
57		72
58		11
59		18
60		5
61		9
62		2
63		9
64		8
65		27
66	0
67		65
68		8
69		10
70		15
71		17
72		12
73	0
74		16
75		1
76		6
77		279
78		7
79		3
80		7
81		7
82		1
83		3
84		12
85		28
86		10
87		2
88		9
89		4

^aFrom the female patients with IIA stage, we were able to expand CTCs in cell culture