Fig. 6
From: SOX2 interferes with the function of CDX2 in bile acid-induced gastric intestinal metaplasia
miR-21 was increased in bile acid-stimulated gastric cells and IM. a The expression of miR-21 in 6 gastric cell lines. b, c The expression of miR-21 was increased upon stimulation with DCA in a dose-dependent manner in GES-1 and AZ-521 cells. Dose: 50, 100, 150 and 200 μM for 24 h. d The expression level of miR-21 in normal gastric tissues and gastric intestinal metaplasia tissues; scale bars: 200 μm (top) and 100 μm (bottom). e qRT-PCR of miR-21 levels in 8 pairs of matched human IM specimens. Each symbol represents mean value of an individual patient. f Western blotting results indicated that the inhibition of SOX2 in bile acid-treated AGS cells was partially rescued in miR-21 knockdown AGS cells. *P < 0.05; **P < 0.01; NS not significant (P > 0.05)