Fig. 3

CIB1 depletion sensitizes MDA-436 TNBC but not normal ME16C cells to TRAIL. Combination of CIB1 depletion with TRAIL in both TNBC and normal cells was tested. Cells were infected with control or CIB1 shRNA for 2 days before the addition of vehicle (water) or the indicated concentrations of a death receptor ligand TRAIL for 48 h. Percent cell death quantified via trypan blue exclusion using a MDA-436 TNBC (n = 4) and b ME16C normal breast epithelial cell lines (n = 3). Data represent mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, ANOVA). CIB1 depletion plus TRAIL enhanced caspase-8 and -3 activation. Representative Western blot for pro-caspase-8, cleaved caspase-8, pro-caspase-9, cleaved caspase-9, cleaved caspase-3, CIB1, and vinculin in c MDA-436 TNBC (n = 3) and d ME16C cells (n = 3)