Fig. 3

LncRNA XIST knockdown suppressed Hela and C3AA cell proliferation in vitro. a Relative expression of XIST after Hela and C3AA cells transfected with XIST, si-XIST or empty vector. It showed that the inhibition effect of si-XIST2 was obvious. The positive effect of XIST on cell proliferation of Hela and C3AA cells was detected by CCK-8 assay (b, c) and EdU assay (d, e). f, g Effect of XIST on cell cycle of Hela and C3AA cells was determined with flow cytometry. The results revealed that downregulation of XIST blocked cell cycle in G0 phase. h, i Overexpression of XIST led to the decreased cell apoptosis rate of Hela and C3AA cells detected by flow cytometry. j, k Hela and C3AA cells were transfected with XIST and si-XIST and subject to western blot analysis using antibodies against c-caspase3, Bcl-2, total PARP, cleaved PARP with GAPDH as a loading control. *P < 0.05, **P < 0.01, compared with NC