Fig. 4

LncRNA XIST inhibition suppressed tumor growth in vivo. a Tumor growth curves determined after injection of Hela cells transfected with si-XIST or RNAi vector. The tumor volume was calculated every 7 days from 2 to 7 weeks. b Tumor weight of nude mouse was measured at the end of 49 days. c Photographs of tumors excised 7 weeks after si-XIST transfected Hela cells injected into nude mice. It was indicated that si-XIST inhibited tumor growth. d–g Photomicrograph showing immunohistochemical staining for Ki-67, E-cadherin and vimentin. Bar = 200 μm. The RNA expression of XIST (h), miR-140-5p (i) and ORC1 (j) were determined by qRT-PCR after 7 weeks. Si-XIST increased the levels of E-cadherin and miR-140-5p, but decreased Ki-67, vimentin, XIST and ORC1 expression levels. *P < 0.05, **P < 0.01, compared with control