Fig. 5

LncRNA XIST directly interacted with miR-140-5p. a Sequence alignment of miR-140-5p with the putative binding sites with in the wild-type regions of XIST. b Targeting relationship between miR-140-5p and ORC1 in Hela cells was confirmed by dual-luciferase reporter assay. The luciferase activity was significantly reduced after co-transfection with miR-140-5p mimics and XIST-wt. c, d MS2-RIP followed by miRNA RT-PCR to detect endogenous miR-140-5p associated with the MS2-tagged XIST transcript. e QRT-PCR analysis of miR-140-5p expression in Hela and C3AA cells transfected with si-XIST or si-NC. f Relative expression of miR-140-5p in Hela and C3AA cells transfected with miR-140-5p mimics or inhibitor was quantified by qRT-PCR, which showed miR-140-5p was upregulated by miR-140-5p mimics and downregulated by miR-140-5p inhibitor. g XIST expression in Hela and C3AA cells was no change when the cells were transfected with miR-140-5p inhibitor or miR-NC. h The expression of miR-140-5p was determined by qRT-PCR in 30 pairs of cervical cancer tissues (T) compared with adjacent non-tumor tissues (N). It was showed that miR-140-5p was downregulated in cervical cancer tissues. “n” indicates sample number. **P < 0.01, ***P < 0.001, compared with miR-NC/MS2/si-NC/NC