Table 2 Primer sequences for qRT-PCR
Gene | Sequences |
|---|---|
XIST | |
Forward sequence | 5′-AATGACTGACCACTGCTGGG-3′ |
Reverse sequence | 5′-GTGTAGGTGGTTCCCCAAGG-3′ |
hsa-miR-140-5p | |
Forward sequence | 5′-CAGUGGUUUUACCCU-3′ |
Reverse sequence | 5′-TGGTGTCGTGGAGTCG-3′ |
ORC1 | |
Forward sequence | 5′-GTCCAATGTTGTAGCCGTGC-3′ |
Reverse sequence | 5′-CGACGCTGAGATGGGATTGT-3′ |
GAPDH | |
Forward sequence | 5′-CAAGGTCATCCATGACAACTTTG-3′ |
Reverse sequence | 5′-GTCCACCACCCTGTTGCTGTAG-3′ |
U6 | |
Forward sequence | 5′-CTCGCTTCGGCAGCACA-3′ |
Reverse sequence | 5′-AACGCTTCACGAATTTGCGT-3′ |