Fig. 2

MiR-208a-5p is a metastatic suppressor and downregulates DAAM1. a DAAM1 gene structure and A/G polymorphism in the DAAM1 3′-UTR at the miR-208a-5p binding sites. b The miR-208a-5p expression in lymphnode metastatic BrCa tissues and non-metastatic BrCa tissues. N0, without lymphnode metastasis. N1–N3, with lymphnode metastasis. c MiR-208a-5p exhibited the lower expression level in MCF-7 and MDA-MB-231 BrCa cells than that in MCF-10A mammary epithelial cells. d The luciferase activity in COS-7 cells transfected with wild type DAAM1 3′-UTR (DAAM1 wild 3′-UTR) and miR-208a-5p or miRNA-Ctrl. e, f The downregulated DAAM1 both in mRNA and protein levels after miR-208a-5p overexpression, and the upregulated DAAM1 protein expression after transfection of miR-208a-5p inhibitor (anti-sense of miR-208a-5p) in MDA-MB-231 and MCF-7 BrCa cells. β-actin was used as the loading control. g Correlation between the expression levels of miR-208a-5p and DAAM1 mRNA in BrCa tissues