Fig. 3

SNP rs79036859 A/G eliminates miR-208a-5p-mediated decrease of DAAM1 expression. a The luciferase activity in COS-7 cells transfected with wild type DAAM1 3′-UTR (DAAM1 wild 3′-UTR) or the mutant of DAAM1 3′-UTR SNP rs79036859 A → G (DAAM1 mutant 3′-UTR) and miR-208a-5p. b The luciferase activity in both MDA-MB-231 and MCF-7 cells transfected with the A allele construct (wild type DAAM1) and the reporter bearing the G allele (mutant DAAM1). c, d The downregulated DAAM1 both in mRNA and protein levels COS-7 cells transfected with wild type DAAM1 3′-UTR (DAAM1 wild 3′-UTR) or the mutant of DAAM1 3′-UTR SNP rs79036859 A → G (DAAM1 mutant 3′-UTR) and miR-208a-5p. The level of active DAAM1 was measured and non-significant difference in ratio of active DAAM1/total DAAM1 were found in above group. β-actin was used as the loading control. e The expression levels of DAAM1 mRNA in BrCa samples with AA, AG, and GG genotypes. f According to the DAAM1 expression using IHC staining, G (AG + GG) genotypes had a higher IRS level of DAAM1 than the wild type AA genotype. g The non-significant difference in miR-208a-5p expression levels were found in wild type AA genotype and mutant G (AG + GG) genotypes in 46 samples