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Fig. 4 | Cancer Cell International

Fig. 4

From: A DAAM1 3′-UTR SNP mutation regulates breast cancer metastasis through affecting miR-208a-5p-DAAM1-RhoA axis

Fig. 4

MiR-208a-5p inhibits the migratory ability of BrCa cells. a, b miR-208a-5p suppressed the migration of MDA-MB-231 and MCF-7 cells, which could be rescued by the overexpression of miR-208a-5p inhibitor (anti-sense of miR-208a-5p) or DAAM1 linking with the G genotype of DAAM1 3′-UTR rs79036859 (DAAM1 mutant 3′-UTR). Migratory cells on the lower-side of Boyden chambers were counted per field of microscope. Bar = 20 μm. Objective lens, magnification, × 20; numerical aperture, 0.75. c, d The overexpression of DAAM1 linking with the G genotype of DAAM1 3′-UTR rs79036859 (DAAM1 mutant 3′-UTR) and DAAM1 lacking 3′-UTR elevated the migration of MDA-MB-231 and MCF-7 cells. DAAM1 linking with wildtype 3′-UTR (DAAM1 WT 3′-UTR) failed to promote the migratory rate of MDA-MB-231 and MCF-7 cells. MDA-MB-231 and MCF-7 cells transfected with DAAM1 WT 3′-UTR, DAAM1 mutant 3′-UTR, or DAAM1 lacking 3′-UTR were examined the migration for 8 h in 8.0-µm porous Boyden chamber membranes

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