Fig. 5

MiR-208a-5p inhibits the RhoA activity and disrupts the formation of microfilament in BrCa cells. a RhoA GTPase activation assays showing the downregulated RhoA activity in MDA-MB-231 and MCF-7 cells after miR-208a-5p overexpression. MiR-208a-5p inhibitor (anti-sense of miR-208a-5p) elevated the RhoA activity in MDA-MB-231 and MCF-7 cells. b miR-208a-5p overexpression disrupted the formation of microfilament in MDA-MB-231 cells. MiR-208a-5p inhibitor (anti-sense of miR-208a-5p) promoted the formation of microfilament. Bar = 10 μm. Objective lens, magnification, ×40; numerical aperture, 0.95. c, d The number of microfilaments in each MDA-MB-231 cell and the length of the longest microfilament were measured (n = 20). e A schematic diagram of DAAM1 (SNP rs79036859) associated signal transduction pathway and miR-208a-5p locating the upstream of DAAM1