Fig. 5

MiR-1180 activates Wnt signal pathway in ovarian cancer cells by targeting SFRP1. a Schematic representation of 3′-UTR of mRNA reporter with the miR-1180 seed-binding sites. b, c Luciferase activity assay of SKOV3 and COC1 cells transfected with luciferase constructs containing WT-3′UTR and Mut-3′UTR of SFRP1. d SFRP2 mRNA was determined by qPCR in SKOV3 and COC1 cells transfected with miR-1180 mimics or their controls. e SFRP1 protein was determined by western blotting in SKOV3 and COC1 cells transfected with miR-1180 mimics or their controls. f Wnt signal pathway associated protein was determined by western blotting in SKOV3 and COC1 cells transfected with miR-1180 mimics or SFRP1. g Protein band density from F. The protein band densities from Wnt5a, β-catenin, c-Myc and cyclinD1 were calculated. h Wnt signal pathway associated protein was determined by western blotting in SKOV3 and COC1 cells treated with the condition medium (CM) from BM-MSCs with miR-1180 mimics transfection. i Protein band density from H. The protein band densities from Wnt5a, β-catenin, c-Myc and cyclinD1 were calculated. **p < 0.01; *p < 0.05