Fig. 3

ITK inhibition reduced invasion and migration in malignant T cell lymphoma cell lines. a, c Jurkat, Hut-78 and H9 cells transfected with vectors carrying ITK shRNA (shITK-34467) or shControl or pretreated with BMS-509744 (3 μM, 5 μM, or 8 μM) were subjected to invasion assays in Transwell chambers coated with Matrigel. b, d Jurkat, Hut-78, and H9 cells transfected with vectors carrying ITK shRNA (shITK-34467) or shControl or pretreated with BMS-509744 (3 μM, 5 μM, or 8 μM) were subjected to migration assays using Transwell chambers. The cells were monitored by a CellTiter-Glo Luminescent Cell Viability Assay. Scale bar 200 μm. e H9, Hut-78 and Jurkat cells were treated with different concentrations of BMS-509744 (3 μM, 5 μM, or 8 μM) for 24 h. CXCR4 expression level were analyzed using real-time PCR. f Cells were treated with indicated concentrations of BMS-509744 for 24 h, then whole cell lysates were detected by western blot for phosphorylated FAK and total RhoA. GAPDH is shown as a loading control. Data are expressed as Mean ± SD and representative of three independent experiments. Statistical analysis was performed using Student’s t test. *P < 0.05, **P < 0.001 compared with the control group