Fig. 4

ITK inhibition induced apoptosis and G2/M phase arrest in malignant T cell lymphoma cells. a, b Jurkat, Hut-78 and H9 cells (2 × 10⁵) were treated with BMS-509744 (3 μM, 5 μM, or 8 μM) for 24 and 48 h, and apoptotic cells were quantified using flow cytometry. c Hut-78, H9 and Jurkat cells (2 × 10⁵) were treated with BMS-509744 (3 μM, 5 μM, or 8 μM) for 48 h. Whole-cell extracts were subjected to Western blot using antibodies directed against caspase-3, PARP, Mcl-1, Bcl-xl, Bcl-2, and Bak. GAPDH is shown as a loading control. d, f Jurkat, Hut-78 and H9 cells (2 × 10⁵) were treated with different concentrations of BMS-509744 (3 μM, 5 μM, or 8 μM) for 24 h, and the cell cycle profiles of the populations were measured using flow cytometry. e Western blot was used to quantify the expression of cell cycle-related proteins (p-cdc2, cdc2 and cyclin B1) after treatment with different concentrations of BMS-509744 (3 μM, 5 μM, or 8 μM) for 48 h. GAPDH is shown as a loading control. Data are expressed as Mean ± SD and representative of three independent experiments. Statistical analysis was performed using Student’s t test. *P < 0.05, **P < 0.001 compared with control group