Fig. 1
From: Contribution of voltage-gated sodium channel β-subunits to cervical cancer cells metastatic behavior
Expression of NaVβs in cervical cancer cell lines. a Total RNA isolated from CeCa cell lines was analyzed by conventional RT-PCR using specific primers for the indicated NaVβ and β-actin as a loading control. Expected fragment sizes for each gene are shown on the right of each panel. M: molecular marker. b Quantitative differences in NaVβs mRNA expression of CeCa cells analyzed by qPCR, normalized with HPRT1 mRNA levels and relative to NaVβ1 in HeLa. Each bar denotes mean ± S.D. of three independent experiments. c Western blot with 100 µg of total protein per lane from each of the cell lines indicated. Specific antibodies against each NaVβ were used and β-actin antibody as a control for loading. d Quantified data for NaVβs protein expression normalized with β-actin expression for each cell line, and relative to NaVβ1 expression in HeLa. Each bar denotes mean ± S.D. of three independent experiments