Fig. 3
From: Contribution of voltage-gated sodium channel β-subunits to cervical cancer cells metastatic behavior
Effect of NaVβs on proliferation and cell-cycle of CeCa cells. Proliferation was evaluated by MTT assays 24, 48, 72 and 96 h post-transfection with plasmids (a) or with specific siRNAs (b) for the β-subunits. The results show the participation of NaVβ1 on proliferation of SiHa cells, as it increases when the protein is overexpressed and decreases when it is downregulated. The other three NaVβs showed no effect on proliferation of SiHa cells, and none of the β-subunits were involved in proliferation of HeLa and CaSki cells. c Cell cycle analysis by flow cytometry in SiHa cells performed 48 h after transfection of synchronized cells with NaVβ1 cDNA or with the specific siRNA against this subunit. Cells were fixed with ice-cold 70% ethanol, stored for at least 24 h at − 20 °C, treated with RNase and stained with PI for 1 h at 37 °C. For each condition, 2 × 104 cells (singlets) were analyzed. Representative histograms of the control mock conditions and the transfected cells (upper panels) of three independent experiments summarized in the bar graphs (bottom panels) as means ± S.D. of the relative percentage of cells in each cell cycle phase. Significance: *P < 0.05, **P < 0.01