Table 1 RT-PCR primers information
From: Contribution of voltage-gated sodium channel β-subunits to cervical cancer cells metastatic behavior
mRNA | GenBank access | Primer pair sequence (5′ → 3′) | PCR Product (bp) | Annealing Temp (°C) |
|---|---|---|---|---|
NaVβ1 | NM_001037 | F: AGAAGGGCACTGAGGAGTTT R: GCAGCGATCTTCTTGTAGCA | 379 | 60 |
NaVβ2 | NM_004588 | F: GCCCACCCGACTAACATCTC R: ATGCGGAACTGGAGGAACA | 285 | 62 |
NaVβ3 | NM_018400 | F: CTGGCTTCTCTCGTGCTTAT R: TCAAACTCCCGGGACACATT | 353 | 60 |
NaVβ4 | NM_174934 | F: CACGCCACCATCTTCCTCCAA R: TGCAGCTGCTCAGCCCGAAG | 284 | 65 |
β-actin | NM_001101 | F: GCTCGTCGTCGACAACGGCTC R: CAAACATGATCTGGGTCATCTTCTC | 353 | 60 |