Fig. 2

miR-182-5p direct targets Cofilin 1 mRNA 3′UTR. a TargetscanHuman 7.2 analysis showed that Cofilin 1 mRNA 3′UTR contains a predicted miR-182-5p binding site at position 135-142. b Wt-Cofilin1-3′UTR sequence complementarity to miR-182-5p at position 135-142, and Mut-Cofilin1-3′UTR has a mutant at miR-182-5p binding site. c Relative luciferase activity was measured after miR-182 mimics, miR-inhibitor, wt-Cofilin 1-3′UTR-psi-CHECK-2 and mut-Cofilin 1-3′UTR-psi-CHECK-2 plasmids transfection. d Cofilin 1 mRNA expression was measured after miR-182 mimics and Cofilin 1 expression plasmids transfection by qRT-PCR. e miR-182 mRNA level was measured in bladder tumor and homologous para-tumor tissues by qRT-PCR. f miR-182 expression level was measured in bladder cancer cell lines (RT4 and T24) and normal bladder epithelial cell line (SV-Huc-1) measured by qRT-PCR. ** Indicates p < 0.01, ***indicates p < 0.001