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Table 1 Features of tissue specimens and established cells

From: Establishment of primary human breast cancer cell lines using “pulsed hypoxia” method and development of metastatic tumor model in immunodeficient mice

Primary culture (method-dependent)

Morphology

Tissue specimens

Collagenase dissociation

Collagenase-free

BrC3

f

e

Bl mammae

T1N0M0

BrC4

f

Bl mammae

T2N0M0

BrC5

f

Bl mammae

T3N0M0

BrC6

f

Bl mammae

T2N1M0

BrCCh2

f

e

Bl mammae

T2N0M0

4 courses of chemotherapy

BrCCh3

f

f

Bl mammae T1N0M0

4 courses of chemotherapy

BrCCh4

e

Bl mammae T4N1M0

4 courses of chemotherapy

BN4

f

Normal cells

  1. 4 courses of chemotherapy included doxorubicin/cyclophosphamide before surgery
  2. f fibroblastoid-like morphology, e epithelial-like morphology