Fig. 1

WM35-R was selected from WM35 for resistance to MDM2 inhibitors. a Growth inhibition curves for the response of WM35 and WM35-R cells to treatment with MDM2 inhibitors for 72 h. Immunobloting of WM35 and WM35-R following treatment with MDM2 inhibitors, RG7388 (b) and HDM201 (c) for 6 h. d CDKN1A, MDM2, PUMA and BAX expressions by qRT-PCR in WM35 and WM35-R following treatment with 0.2 µM RG7388 for 6 and 24 h. e Chromatogram showing p53 mutations in WM35-R. f Mutation specific PCR detecting the presence of a low frequency of mutant alleles in the parental WM35 cell population