Fig. 6

SiRNA mediated knockdown of p53 in WM35 reduced apoptosis after trametinib or vemurafenib treatment. a mRNA expression of p53 after 24 h siRNA mediated knockdown, relative to SiControl and GAPDH control in WM35. b p53 protein levels after siRNA mediated knockdown for 24, 48 and 72 h detected by immumoblotting. SRB assay of growth inhibition for WM35 after 24-h siRNA treatment followed by 72-h trametinib (c) or vemurafenib (d) treatments. e, f Caspase 3/7 activity in WM35 after 24-h siRNA treatment followed by 24-h trametinib (c) or vemurafenib (d) treatments. Statistically significant differences (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) between SiRNA p53 knockdown and SiRNA control treated cells are shown above the bars for each treatment normalised to DMSO control. Data are presented as mean ± standard error of mean (SEM) for three independent repeats